Rabbit anti-Human SHMT1 Polyclonal Antibody | anti-SHMT1 antibody

Anti-SHMT1 Antibody

Cat. Num. AAA8245179

• Gene Names: SHMT1; SHMT; CSHMT
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: SHMT1; Polyclonal Antibody; Anti-SHMT1 Antibody; Serine hydroxymethyltransferase; cytosolic; SHMT; Glycine hydroxymethyltransferase; Serine methylase; anti-SHMT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of SHMT1 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 345

• Applicable Applications for anti-SHMT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1/500 - 1/2000; IHC: 1/50 - 1/200
• Immunogen: Recombinant full length protein of human SHMT1
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of SHMT1 expression in HepG2 (A), MCF7 (B), Hela (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of SHMT1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-SHMT1 antibody

Rabbit polyclonal antibody to SHMT1

NCBI and Uniprot Product Information

• NCBI GI #: 528881075
• NCBI GeneID: 6470
• NCBI Accession #: NP_001268715.1
• NCBI GenBank Nucleotide #: NM_001281786.1
• UniProt Accession #: P34896; Q96HY0; Q9UMD1; Q9UMD2; B4DPM9; D3DXD0
• Molecular Weight: 37,672 Da
• NCBI Official Full Name: serine hydroxymethyltransferase, cytosolic isoform 3
• NCBI Official Synonym Full Names: serine hydroxymethyltransferase 1
• NCBI Official Symbol: SHMT1
• NCBI Official Synonym Symbols: SHMT; CSHMT
• NCBI Protein Information: serine hydroxymethyltransferase, cytosolic
• UniProt Protein Name: Serine hydroxymethyltransferase, cytosolic
• Protein Family: Serine hydroxymethyltransferase
• UniProt Gene Name: SHMT1
• UniProt Synonym Gene Names: SHMT
• UniProt Entry Name: GLYC_HUMAN

NCBI Description

This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Mitochondrial; Methyltransferase; Amino Acid Metabolism - glycine, serine and threonine; EC 2.1.2.1; Other Amino Acids Metabolism - cyanoamino acid; Energy Metabolism - methane; Cofactor and Vitamin Metabolism - one carbon pool by folate

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: glycine hydroxymethyltransferase activity; L-allo-threonine aldolase activity; protein binding; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: carnitine biosynthetic process; dTMP biosynthetic process; folic acid metabolic process; glycine biosynthetic process from serine; glycine metabolic process; L-serine catabolic process; L-serine metabolic process; protein homotetramerization; protein tetramerization; purine base biosynthetic process; tetrahydrofolate metabolic process