Human Centromere Protein E (CENPE) ELISA Kit | CENPE elisa kit

Human Centromere Protein E (CENPE) ELISA Kit

Cat. Num. AAA2019092

• Gene Names: CENPE; KIF10; CENP-E; MCPH13; PPP1R61
• Reactivity: Human
• Synonyms: Centromere Protein E (CENPE); Human Centromere Protein E (CENPE) ELISA Kit; CENP-E; KIF10; Centromere-Associated Protein E; CENPE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Centromere Protein E (CENPE).; No significant cross-reactivity or interference between Centromere Protein E (CENPE) and analogues was observed.
• Sequence Length: 2665

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Centromere Protein E (CENPE) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Centromere Protein E (CENPE) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CENPE elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein E (CENPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Centromere Protein E (CENPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein E (CENPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Centromere Protein E (CENPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CENPE elisa kit

Signal transduction; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 119626574
• NCBI GeneID: 1062
• UniProt Accession #: Q02224; Q4LE75; A6NKY9; A8K2U7
• Molecular Weight: 301,789 Da
• NCBI Official Full Name: centromere protein E, 312kDa, isoform CRA_a
• NCBI Official Synonym Full Names: centromere protein E, 312kDa
• NCBI Official Symbol: CENPE
• NCBI Official Synonym Symbols: KIF10; CENP-E; MCPH13; PPP1R61
• NCBI Protein Information: centromere-associated protein E
• UniProt Protein Name: Centromere-associated protein E
• Protein Family: Centromere-associated protein
• UniProt Gene Name: CENPE
• UniProt Synonym Gene Names: CENP-E
• UniProt Entry Name: CENPE_HUMAN

NCBI Description

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 4q24-q25

Cellular Component: microtubule cytoskeleton; kinetochore; microtubule; kinesin complex; membrane; cytoplasm; outer kinetochore of condensed chromosome; chromosome; midbody; nucleus; cytosol; chromosome, pericentric region

Molecular Function: kinetochore binding; protein binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: kinetochore assembly; metabolic process; multicellular organismal development; regulation of mitotic metaphase/anaphase transition; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; microtubule-based movement; chromosome segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; positive regulation of protein kinase activity; mitotic cell cycle; blood coagulation

Disease: Microcephaly 13, Primary, Autosomal Recessive