Western Blot (WB)
(Anti- CD244 antibody, MBS176259, Western blottingAll lanes: Anti CD244 (MBS176259) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Rabbit anti-Human CD244 Polyclonal Antibody | anti-CD244 antibody
Anti-CD244 antibody
Gene Names
CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
CD244; Polyclonal Antibody; Anti-CD244 antibody; Natural killer cell receptor 2B4; CD244 molecule; natural killer cell receptor 2B4; 2B4 antibody; C9.1 antibody; CD244 antibody; CD244 antigen antibody; CD244 molecule natural killer cell receptor 2B4 antibody; CD244 natural killer cell receptor 2B4 antibody; CD244_HUMAN antibody; F730046O15Rik antibody; h2B4 antibody; Ly90 antibody; NAIL antibody; Natural killer cell activation-inducing ligand antibody; Natural killer cell receptor 2B4 antibody; NK cell activation inducing ligand antibody; NK cell activation inducing ligand NAIL antibody; NK cell activation-inducing ligand antibody; NK cell type I receptor protein 2B4 antibody; NKR2B4 antibody; Nmrk antibody; Non-MHC restricted killing associated antibody; OTTHUMP00000027884 antibody; p38 antibody; SLAM family; member 4 antibody; SLAMF4 antibody; anti-CD244 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
370
Applicable Applications for anti-CD244 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD244 (357-370aa RLSRKELENFDVYS).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- CD244 antibody, MBS176259, Western blottingAll lanes: Anti CD244 (MBS176259) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Western Blot (WB)
(Anti- CD244 antibody, MBS176259, Western blottingAll lanes: Anti CD244 (MBS176259) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Immunohistochemistry (IHC)
(Anti- CD244 antibody, MBS176259, IHC(P)IHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti- CD244 antibody, MBS176259, IHC(P)IHC(P): Human Tonsil Tissue )
Related Product Information for anti-CD244 antibody
Description: Rabbit IgG polyclonal antibody for Natural killer cell receptor 2B4(CD244) detection. Tested with WB, IHC-P in Human.
Background: CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al.(1999) mapped the 2B4 gene to 1q22. Suzuki et al.(2008) identified a functional single-nucleotide polymorphism(SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al.(1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al.(1998) demonstrated that CD48 binds to 2B4. Watzl et al.(2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.
Background: CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al.(1999) mapped the 2B4 gene to 1q22. Suzuki et al.(2008) identified a functional single-nucleotide polymorphism(SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al.(1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al.(1998) demonstrated that CD48 binds to 2B4. Watzl et al.(2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,784 Da
NCBI Official Full Name
Natural killer cell receptor 2B4
NCBI Official Synonym Full Names
CD244 molecule, natural killer cell receptor 2B4
NCBI Official Symbol
CD244
NCBI Official Synonym Symbols
2B4; NAIL; Nmrk; NKR2B4; SLAMF4
NCBI Protein Information
natural killer cell receptor 2B4; h2B4; SLAM family member 4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL; signaling lymphocytic activation molecule 4
UniProt Protein Name
Natural killer cell receptor 2B4
Protein Family
UniProt Gene Name
CD244
UniProt Synonym Gene Names
2B4; NAIL; NKR2B4; h2B4; SLAMF4
UniProt Entry Name
CD244_HUMAN
NCBI Description
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Uniprot Description
CD244: Modulate other receptor-ligand interactions to enhance leukocyte activation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: signal transduction; blood coagulation; leukocyte migration
Disease: Rheumatoid Arthritis
Research Articles on CD244
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Product Notes
The CD244 cd244 (Catalog #AAA176259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD244 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD244 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the CD244 cd244 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD244, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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