Typical Testing Data/Standard Curve (for reference only)
Human CD180 antigen (CD180) ELISA Kit | CD180 elisa kit
Human CD180 antigen (CD180) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CD180 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD180 antibody and an CD180-HRP conjugate. The assay sample and buffer are incubated together with CD180-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD180 concentration since CD180 from samples and CD180-HRP conjugate compete for the anti-CD180 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD180 from the sample, fewer sites are left to bind CD180-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD180 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]
Uniprot Description
CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q12
Cellular Component: plasma membrane; integral to membrane
Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response
Research Articles on CD180
Similar Products
Product Notes
The Human CD180 cd180 (Catalog #AAA7203146) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7203146 ELISA Kit recognizes Human CD180. It is sometimes possible for the material contained within the vial of "CD180 antigen (CD180), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
