Rabbit NOS III Polyclonal Antibody | anti-NOS3 antibody

NOS III, CT (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial) (PE)

Cat. Num. AAA6490318

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Human, Bovine
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NOS III; Polyclonal Antibody; CT (Nitric Oxide Synthase 3; Nitric Oxide Synthase 3 Endothelial Cell; NOS Type III; NOS3; Endothelial Nitric Oxide Synthase 3; Endothelial NOS; eNOS; ECNOS; EC-NOS; Constitutive NOS; cNOS; Nitric Oxide Synthase Endothelial) (PE); anti-NOS3 antibody

• Host: Rabbit
• Reactivity: Human, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human eNOS/NOS III, Mr ~135kD. Species Crossreactivity: bovine.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1203

• Applicable Applications for anti-NOS3 antibody: Western Blot (WB)
• Application Notes: WB: 0.5-2ug/ml detects eNOS/NOS III in RIPA lysates from HUVEC cells.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa1181-1194, C-QERQLRGAVPWAFD of human eNOS/NOS III, KLH conjugated. Species sequence homology: mouse, guinea pig, porcine, rat and canine; 10/11aa.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(HUVEC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N5350-22 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates eNOS/NOS III (135kD).)

Product Categories/Family for anti-NOS3 antibody

Antibodies; Nitric Oxide

References

1. Llorens, S., et al., Biogerontology 8: 663-672 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 40254422
• NCBI GeneID: 4846
• NCBI Accession #: NP_000594
• NCBI GenBank Nucleotide #: NP_000594.2
• UniProt Accession #: P29474
• Molecular Weight: 135
• NCBI Official Full Name: nitric oxide synthase, endothelial isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; negative regulation of hydrolase activity; regulation of blood pressure; regulation of the force of heart contraction by chemical signal; lipopolysaccharide-mediated signaling pathway; angiogenesis; negative regulation of blood pressure; regulation of blood vessel size; nitric oxide biosynthetic process; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic