Human CCL1 ELISA Kit | CCL1 elisa kit

Human CCL1 PicoKine ELISA Kit

Cat. Num. AAA176531

• Gene Names: CCL1; P500; SISe; TCA3; I-309; SCYA1
• Reactivity: Human
• Synonyms: CCL1; Human CCL1 PicoKine ELISA Kit; C-C motif chemokine 1; chemokine (C-C motif) ligand 1; BF534335; C C motif chemokine 1; Ccl1; CCL1_HUMAN; CCR8 ligand; Chemokine CC Motif Ligand 1; Inflammatory cytokine I-309; P500; RP23-446K18.2; SCYA1; SISe; Small inducible cytokine A1; Small-inducible cytokine A1; T lymphocyte secreted protein I-309; T lymphocyte-secreted protein I-309; TCA3; CCL1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL1
• Sequence Length: 96

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 4pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CCL1 PicoKine ELISA Kit standard curve )

Related Product Information for CCL1 elisa kit

Principle of the assay: human CCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL1 has been precoated onto 96-well plates. Standards(NSO, K24-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL1 amount of sample captured in plate.
Background: CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

NCBI and Uniprot Product Information

• NCBI GI #: 123950
• NCBI GeneID: 6346
• UniProt Accession #: P22362; Q2M309; B2R5G9
• Molecular Weight: 10,992 Da
• NCBI Official Full Name: C-C motif chemokine 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 1
• NCBI Official Symbol: CCL1
• NCBI Official Synonym Symbols: P500; SISe; TCA3; I-309; SCYA1
• NCBI Protein Information: C-C motif chemokine 1; inflammatory cytokine I-309; T lymphocyte-secreted protein I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
• UniProt Protein Name: C-C motif chemokine 1
• Protein Family: Cyclin
• UniProt Gene Name: CCL1
• UniProt Synonym Gene Names: SCYA1
• UniProt Entry Name: CCL1_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL1: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; viral reproduction; immune response; signal transduction; chemotaxis