Human CBR1 (Carbonyl reductase [NADPH] 1) ELISA Kit | CBR1 elisa kit

Human CBR1 (Carbonyl reductase [NADPH] 1) ELISA Kit

Cat. Num. AAA7612219

• Gene Names: CBR1; CBR; hCBR1; SDR21C1
• Reactivity: Human
• Synonyms: CBR1 (Carbonyl reductase [NADPH] 1); Human CBR1 (Carbonyl reductase [NADPH] 1) ELISA Kit; Carbonyl reductase [NADPH] 1; 15-hydroxyprostaglandin dehydrogenase [NADP(+)]; 20-beta-hydroxysteroid dehydrogenase; NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; PG-9-KR; Prostaglandin-E(2) 9-reductase; Short chain dehydrogenase/reductase family 21C member 1; CBR; CRN; SDR21C1; CBR1; CBR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CBR1. No significant cross-reactivity or interference between CBR1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CBR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502599
• NCBI GeneID: 873
• NCBI Accession #: NP_001748.1
• NCBI GenBank Nucleotide #: NM_001757.2
• UniProt Accession #: P16152; Q3LHW8; B2RBZ7
• Molecular Weight: 30,375 Da
• NCBI Official Full Name: carbonyl reductase
• NCBI Official Synonym Full Names: carbonyl reductase 1
• NCBI Official Symbol: CBR1
• NCBI Official Synonym Symbols: CBR; hCBR1; SDR21C1
• NCBI Protein Information: carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase
• UniProt Protein Name: Carbonyl reductase [NADPH] 1
• Protein Family: Carbonyl reductase
• UniProt Gene Name: CBR1
• UniProt Synonym Gene Names: CBR; CRN
• UniProt Entry Name: CBR1_HUMAN

NCBI Description

Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q. [provided by RefSeq, Jul 2008]

Uniprot Description

CBR1: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.1.1.189; EC 1.1.1.197; EC 1.1.1.184; Oxidoreductase

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: cytosol; vesicle

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity; 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity

Biological Process: epithelial cell differentiation; vitamin K metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; drug metabolic process