Human NK6 Homeobox Protein 1 (NKX6-1) RTU ELISA Kit | NKX6-1 rtu elisa kit

Human NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit

Cat. Num. AAA4501386

• Gene Names: NKX6-1; NKX6A; NKX6.1
• Reactivity: Human
• Synonyms: NK6 Homeobox Protein 1 (NKX6-1); Human NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit; NKX6A; Nkx6.1; NK6 Transcription Factor Related; Locus 1; Homeobox protein NK-6 homolog A; NKX6-1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 367

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.096ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NKX6-1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKX6-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NKX6-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKX6-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NKX6-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 111120318
• NCBI GeneID: 4825
• NCBI Accession #: NP_006159.2
• NCBI GenBank Nucleotide #: NM_006168.2
• UniProt Accession #: P78426
• Molecular Weight: 37,849 Da
• NCBI Official Full Name: homeobox protein Nkx-6.1
• NCBI Official Synonym Full Names: NK6 homeobox 1
• NCBI Official Symbol: NKX6-1
• NCBI Official Synonym Symbols: NKX6A; NKX6.1
• NCBI Protein Information: homeobox protein Nkx-6.1
• UniProt Protein Name: Homeobox protein Nkx-6.1
• Protein Family: Homeobox protein
• UniProt Gene Name: NKX6-1
• UniProt Synonym Gene Names: NKX6A

NCBI Description

In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008]

Uniprot Description

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals ().