Typical Testing Data/Standard Curve (for reference only)
Human Calpastatin (CAST) ELISA Kit | CAST elisa kit
Human Calpastatin (CAST) ELISA Kit
Gene Names
ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
Reactivity
Human
Synonyms
Calpastatin (CAST); Human Calpastatin (CAST) ELISA Kit; BS17; Calpain inhibitor; Sperm BS-17 component; CAST elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Calpastatin (CAST).
No significant cross-reactivity or interference between Calpastatin (CAST) and analogues was observed.
No significant cross-reactivity or interference between Calpastatin (CAST) and analogues was observed.
Sequence Length
1371
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.33ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpastatin (CAST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpastatin (CAST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpastatin (CAST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CAST elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpastatin (CAST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpastatin (CAST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpastatin (CAST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpastatin (CAST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 13
NCBI Official Symbol
ADAMTS13
NCBI Official Synonym Symbols
VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 13
Protein Family
UniProt Gene Name
ADAMTS13
UniProt Synonym Gene Names
C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
NCBI Description
This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
Research Articles on CAST
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Product Notes
The Human CAST adamts13 (Catalog #AAA2707580) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707580 ELISA Kit recognizes Human CAST. It is sometimes possible for the material contained within the vial of "Calpastatin (CAST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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