Human CA2 ELISA Kit | CA2 elisa kit

Human CA2 (Carbonic Anhydrase II) ELISA Kit

Cat. Num. AAA2504788

• Gene Names: CA2; CAC; CAII; Car2; CA-II; HEL-76
• Reactivity: Human
• Synonyms: CA2; Human CA2 (Carbonic Anhydrase II) ELISA Kit; CA2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human CA2 in samples. No significant cross-reactivity or interference between Human CA2 and analogues was observed.
• Sequence Length: 260

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.13-200ng/mL
• Sensitivity: 1.88ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CA2 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CA2 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CA2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CA2. You can calculate the concentration of Human CA2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49456705
• NCBI GeneID: 760
• UniProt Accession #: P00918; Q6FI12; Q96ET9; B2R7G8
• Molecular Weight: 29,246 Da
• NCBI Official Full Name: CA2, partial
• NCBI Official Synonym Full Names: carbonic anhydrase II
• NCBI Official Symbol: CA2
• NCBI Official Synonym Symbols: CAC; CAII; Car2; CA-II; HEL-76
• NCBI Protein Information: carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76
• UniProt Protein Name: Carbonic anhydrase 2
• Protein Family: Beta-carotene hydroxylase
• UniProt Gene Name: CA2
• UniProt Synonym Gene Names: CAC; CA-II
• UniProt Entry Name: CAH2_HUMAN

NCBI Description

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; basolateral plasma membrane; apical part of cell; axon; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to zinc ion; bicarbonate transport; response to estrogen stimulus; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3