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Typical Testing Data/Standard Curve (for reference only)

Sheep Carbonic Anhydrase II ELISA Kit | CA2 elisa kit

Sheep Carbonic Anhydrase II ELISA Kit

Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76
Reactivity
Sheep
Synonyms
Carbonic Anhydrase II; Sheep Carbonic Anhydrase II ELISA Kit; CA2 elisa kit
Ordering
For Research Use Only!
Reactivity
Sheep
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Intended Uses
This CA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CA2. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CA2 elisa kit
Principle of the Assay: CA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA2 antibody and an CA2-HRP conjugate. The assay sample and buffer are incubated together with CA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA2 concentration since CA2 from samples and CA2-HRP conjugate compete for the anti-CA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA2 from the sample, fewer sites are left to bind CA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
760
UniProt Accession #
Molecular Weight
29,246 Da
NCBI Official Full Name
CA2, partial
NCBI Official Synonym Full Names
carbonic anhydrase II
NCBI Official Symbol
CA2
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76
NCBI Protein Information
carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76
UniProt Protein Name
Carbonic anhydrase 2
Protein Family
UniProt Gene Name
CA2
UniProt Synonym Gene Names
CAC; CA-II
UniProt Entry Name
CAH2_HUMAN

NCBI Description

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; basolateral plasma membrane; axon; apical part of cell; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to estrogen stimulus; response to zinc ion; bicarbonate transport; regulation of intracellular pH; morphogenesis of an epithelium; positive regulation of bone resorption; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3

Research Articles on CA2

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Product Notes

The Sheep CA2 ca2 (Catalog #AAA736236) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736236 ELISA Kit recognizes Sheep CA2. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase II, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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