Human BMP Binding Endothelial Regulator (BMPER) ELISA Kit | BMPER elisa kit

Human BMP Binding Endothelial Regulator (BMPER) ELISA Kit

Cat. Num. AAA2706709

• Gene Names: BMPER; CV2; CV-2; CRIM3
• Reactivity: Human
• Synonyms: BMP Binding Endothelial Regulator (BMPER); Human BMP Binding Endothelial Regulator (BMPER) ELISA Kit; Cv2; CRIM3; Crossveinless-2; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; BMPER elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BMP Binding Endothelial Regulator (BMPER).; No significant cross-reactivity or interference between BMP Binding Endothelial Regulator (BMPER) and analogues was observed.
• Sequence Length: 685

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BMP Binding Endothelial Regulator (BMPER) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BMP Binding Endothelial Regulator (BMPER) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMPER elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP Binding Endothelial Regulator (BMPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BMP Binding Endothelial Regulator (BMPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMP Binding Endothelial Regulator (BMPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMP Binding Endothelial Regulator (BMPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37693519
• NCBI GeneID: 168667
• NCBI Accession #: NP_597725.1
• NCBI GenBank Nucleotide #: NM_133468.4
• UniProt Accession #: Q8N8U9; Q8TF36; A8K1P8
• Molecular Weight: 75,997 Da
• NCBI Official Full Name: BMP-binding endothelial regulator protein
• NCBI Official Synonym Full Names: BMP binding endothelial regulator
• NCBI Official Symbol: BMPER
• NCBI Official Synonym Symbols: CV2; CV-2; CRIM3
• NCBI Protein Information: BMP-binding endothelial regulator protein
• UniProt Protein Name: BMP-binding endothelial regulator protein
• Protein Family: BMP-binding endothelial regulator protein
• UniProt Gene Name: BMPER
• UniProt Synonym Gene Names: KIAA1965; hCV2

NCBI Description

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis. [provided by RefSeq, Dec 2011]

Uniprot Description

Inhibitor of bone morphogenetic protein (BMP) function, it may regulate BMP responsiveness of osteoblasts and chondrocytes.