Rabbit anti-Mouse Sgk196 Polyclonal Antibody | anti-Sgk196 antibody

Sgk196, CT (Sgk196, Protein kinase-like protein SgK196, Sugen kinase 196) (Azide free) (HRP)

Cat. Num. AAA6346926

• Gene Names: Pomk; Sgk196; 4930444A02Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Sgk196; Polyclonal Antibody; CT (Sgk196; Protein kinase-like protein SgK196; Sugen kinase 196) (Azide free) (HRP); Sugen kinase 196; anti-Sgk196 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Sgk196 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Sgk196 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of Mouse Sgk196.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Sgk196 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Sgk196 antibody detected the Sgk196 protein (arrow).)

Related Product Information for anti-Sgk196 antibody

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.

Product Categories/Family for anti-Sgk196 antibody

Antibodies; Protein Kinases

References

Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 21312828
• NCBI GeneID: 74653
• NCBI Accession #: NP_083313.1
• NCBI GenBank Nucleotide #: NM_029037.4
• UniProt Accession #: Q3TUA9; Q3TBZ0; Q8BZ83; Q8R2S2; Q9D5G4
• Molecular Weight: 39,969 Da
• NCBI Official Full Name: protein O-mannose kinase
• NCBI Official Synonym Full Names: protein-O-mannose kinase
• NCBI Official Symbol: Pomk
• NCBI Official Synonym Symbols: Sgk196; 4930444A02Rik
• NCBI Protein Information: protein O-mannose kinase
• UniProt Protein Name: Protein O-mannose kinase
• UniProt Gene Name: Pomk
• UniProt Synonym Gene Names: Sgk196; POMK

Uniprot Description

Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif ().