Human ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) ELISA Kit | ATP7b elisa kit

Human ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) ELISA Kit

Cat. Num. AAA2024213

• Gene Names: ATP7B; WD; PWD; WC1; WND
• Reactivity: Human
• Synonyms: ATPase; Cu++ Transporting Beta Polypeptide (ATP7b); Human ATPase; Cu++ Transporting Beta Polypeptide (ATP7b) ELISA Kit; PWD; WC1; WD; WND; Wilson Disease Protein; Copper pump 2; Wilson disease-associated protein; ATP7b elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP7b. No significant cross-reactivity or interference between ATP7b and analogues was observed.
• Sequence Length: 1,447

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP7b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP7b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP7b elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATP7b in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ATP7b. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ATP7b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP7b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP7b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ATP7b elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119629300
• NCBI GeneID: 540
• UniProt Accession #: P35670; Q16318; Q16319; Q4U3V3; Q59FJ9; Q5T7X7
• Molecular Weight: 155,125 Da
• NCBI Official Full Name: ATPase, Cu++ transporting, beta polypeptide, isoform CRA_d
• NCBI Official Synonym Full Names: ATPase, Cu++ transporting, beta polypeptide
• NCBI Official Symbol: ATP7B
• NCBI Official Synonym Symbols: WD; PWD; WC1; WND
• NCBI Protein Information: copper-transporting ATPase 2
• UniProt Protein Name: Copper-transporting ATPase 2
• UniProt Gene Name: ATP7B
• UniProt Synonym Gene Names: PWD; WC1; WND
• UniProt Entry Name: ATP7B_HUMAN

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, multi-pass; EC 3.6.3.54; Vesicle; Hydrolase; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi membrane; membrane; mitochondrion; basolateral plasma membrane; integral to plasma membrane; perinuclear region of cytoplasm; late endosome; cytoplasmic membrane-bound vesicle; trans-Golgi network

Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding

Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; cellular zinc ion homeostasis; sequestering of calcium ion; copper ion import; copper ion transport; transmembrane transport; copper ion export; intracellular copper ion transport

Disease: Wilson Disease