Rabbit anti-Human CEP55 Polyclonal Antibody | anti-CEP55 antibody

CEP55 polyclonal antibody

Cat. Num. AAA3001937

• Gene Names: CEP55; CT111; URCC6; C10orf3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CEP55; Polyclonal Antibody; CEP55 polyclonal antibody; C10orf3; URCC6; Centrosomal protein of 55 kDa; Cep55; Up-regulated in colon cancer 6; anti-CEP55 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: CEP55 Polyclonal Antibody detects endogenous levels of CEP55 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-CEP55 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human CEP55. AA range: 81-130
• Function: Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
• PTM: There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
• Subcellular Location: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
• Subunit: Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding. Interacts with FAM125A, VPS37B, VPS37C and VPS28.
• Tissue Specificity: Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using CEP55 Polyclonal Antibody)

Western Blot (WB)

(Western blot analysis of the lysates from COLO205 cells using CEP55 antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 187608545
• NCBI GeneID: 55165
• NCBI Accession #: NP_001120654.1
• NCBI GenBank Nucleotide #: NM_001127182.1
• UniProt Accession #: Q53EZ4; Q32WF5; Q3MV20; Q5VY28; Q6N034; Q96H32; Q9NVS7; B2RDG8
• Molecular Weight: 54,178 Da
• NCBI Official Full Name: centrosomal protein of 55 kDa
• NCBI Official Synonym Full Names: centrosomal protein 55kDa
• NCBI Official Symbol: CEP55
• NCBI Official Synonym Symbols: CT111; URCC6; C10orf3
• NCBI Protein Information: centrosomal protein of 55 kDa; cancer/testis antigen 111; up-regulated in colon cancer 6
• UniProt Protein Name: Centrosomal protein of 55 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP55
• UniProt Synonym Gene Names: C10orf3; URCC6; Cep55
• UniProt Entry Name: CEP55_HUMAN

Uniprot Description

CEP55: Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: centriole; centrosome; intercellular bridge; membrane; midbody; cleavage furrow

Molecular Function: protein binding

Biological Process: establishment of protein localization; cell separation during cytokinesis; cytokinesis after mitosis; mitotic metaphase plate congression; nuclear organization and biogenesis