Human ASL (Argininosuccinate lyase) ELISA Kit | ASL elisa kit

Human ASL (Argininosuccinate lyase) ELISA Kit

Cat. Num. AAA7612307

• Gene Names: ASL; ASAL
• Reactivity: Human
• Synonyms: ASL (Argininosuccinate lyase); Human ASL (Argininosuccinate lyase) ELISA Kit; Argininosuccinate lyase; ASAL; Arginosuccinase; ASL; ASL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ASL. No significant cross-reactivity or interference between ASL and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASL elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 124028641
• NCBI GeneID: 435
• UniProt Accession #: P04424; Q6LDS5; Q96HS2; E7EMI0; E9PE48
• Molecular Weight: 464
• NCBI Official Full Name: Argininosuccinate lyase
• NCBI Official Synonym Full Names: argininosuccinate lyase
• NCBI Official Symbol: ASL
• NCBI Official Synonym Symbols: ASAL
• NCBI Protein Information: argininosuccinate lyase; arginosuccinase; argininosuccinase
• UniProt Protein Name: Argininosuccinate lyase
• UniProt Gene Name: ASL
• UniProt Synonym Gene Names: ASAL
• UniProt Entry Name: ARLY_HUMAN

NCBI Description

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ASL: Defects in ASL are the cause of arginosuccinic aciduria (ARGINSA). An autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Protein type: Lyase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 4.3.2.1; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: cytoplasm; cytosol

Molecular Function: argininosuccinate lyase activity

Biological Process: arginine catabolic process; arginine biosynthetic process via ornithine; internal protein amino acid acetylation; urea cycle

Disease: Argininosuccinic Aciduria