Human Phenol Sulfotransferase (PST) ELISA Kit | PST elisa kit

Human Phenol Sulfotransferase (PST) ELISA Kit

Cat. Num. AAA2024025

• Gene Names: SULT1A1; PST; STP; STP1; P-PST; ST1A1; ST1A3; TSPST1; HAST1/HAST2
• Reactivity: Human
• Synonyms: Phenol Sulfotransferase (PST); Human Phenol Sulfotransferase (PST) ELISA Kit; SULT1A1; HAST1/HAST2; ST1A3; STP1; TSPST1; Sulfotransferase 1A1; Aryl sulfotransferase 1; Phenol sulfotransferase 1; Thermostable phenol sulfotransferase; PST elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phenol Sulfotransferase (PST).; No significant cross-reactivity or interference between Phenol Sulfotransferase (PST) and analogues was observed.
• Sequence Length: 217

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phenol Sulfotransferase (PST) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phenol Sulfotransferase (PST) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PST elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phenol Sulfotransferase (PST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phenol Sulfotransferase (PST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phenol Sulfotransferase (PST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phenol Sulfotransferase (PST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PST elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 553649
• NCBI GeneID: 6817
• UniProt Accession #: P50225; Q2NL71; Q86U58; Q92818; Q9BVU6; Q9UGG7
• Molecular Weight: 25,388 Da
• NCBI Official Full Name: phenol sulfotransferase, partial
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• NCBI Official Symbol: SULT1A1
• NCBI Official Synonym Symbols: PST; STP; STP1; P-PST; ST1A1; ST1A3; TSPST1; HAST1/HAST2
• NCBI Protein Information: sulfotransferase 1A1
• UniProt Protein Name: Sulfotransferase 1A1
• UniProt Gene Name: SULT1A1
• UniProt Synonym Gene Names: STP; STP1; ST1A1; P-PST 1; Ts-PST
• UniProt Entry Name: ST1A1_HUMAN

NCBI Description

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SULT1A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N- hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Energy Metabolism - sulfur; EC 2.8.2.1

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; steroid sulfotransferase activity; flavonol 3-sulfotransferase activity; aryl sulfotransferase activity

Biological Process: estrogen metabolic process; flavonoid metabolic process; xenobiotic metabolic process; sulfation; catecholamine metabolic process; amine metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process