Rabbit anti-Human c-Abl Polyclonal Antibody | anti-c-Abl antibody

c-Abl, NT (H246) (ABL, Tyrosine-protein Kinase ABL1, Abelson Murine Leukemia Viral Oncogene Homolog 1, Proto-oncogene c-Abl, p150, ABL1, JTK7) (APC)

Cat. Num. AAA6466228

• Gene Names: ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: c-Abl; Polyclonal Antibody; NT (H246) (ABL; Tyrosine-protein Kinase ABL1; Abelson Murine Leukemia Viral Oncogene Homolog 1; Proto-oncogene c-Abl; p150; ABL1; JTK7) (APC); anti-c-Abl antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABL1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-c-Abl antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ABL1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ABL1 Antibody (N-term H246) in Y79 cell line lysates (35ug/lane). ABL1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-c-Abl antibody

Antibodies; Apoptosis

References

1. Donaldson, L.W., et al., Proc. Natl. Acad. Sci. USA 99(22):14053-14058 (2002). 2. Pisabarro, M.T., et al., J. Mol. Biol. 281(3):513-521 (1998). 3. Nam, H.J., et al., Structure 4(9):1105-1114 (1996). 4. Chissoe, S.L., et al., Genomics 27(1):67-82 (1995). 5. Gosser, Y.Q., et al., Structure 3(10):1075-1086 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 62362414
• NCBI GeneID: 25
• NCBI Accession #: NP_005148
• NCBI GenBank Nucleotide #: NM_005157
• UniProt Accession #: P00519; Q13869; Q13870; Q16133; Q17R61; Q45F09; A3KFJ3
• Molecular Weight: 124,955 Da
• NCBI Official Full Name: tyrosine-protein kinase ABL1 isoform a
• NCBI Official Synonym Full Names: ABL proto-oncogene 1, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL1
• NCBI Official Synonym Symbols: ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
• NCBI Protein Information: tyrosine-protein kinase ABL1; Abelson tyrosine-protein kinase 1; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolo
• UniProt Protein Name: Tyrosine-protein kinase ABL1
• UniProt Gene Name: ABL1
• UniProt Synonym Gene Names: ABL; JTK7
• UniProt Entry Name: ABL1_HUMAN

Uniprot Description

Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; Oncoprotein; EC 2.7.10.2; TK group; Abl family

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; nuclear membrane; extrinsic to internal side of plasma membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; leading edge; nucleolus; nucleus; cytosol; actin cytoskeleton

Molecular Function: protein C-terminus binding; actin monomer binding; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; protein kinase activity; actin filament binding; protein binding; DNA binding; syntaxin binding; protein-tyrosine kinase activity; manganese ion binding; mitogen-activated protein kinase binding; nicotinate-nucleotide adenylyltransferase activity; SH3 domain binding; ATP binding; receptor binding

Biological Process: DNA damage induced protein phosphorylation; positive regulation of apoptosis; cerebellum morphogenesis; negative regulation of BMP signaling pathway; elevation of cytosolic calcium ion concentration; transitional one stage B cell differentiation; B cell receptor signaling pathway; regulation of endocytosis; negative regulation of mitotic cell cycle; cell cycle arrest; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; spleen development; mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of peptidyl-tyrosine phosphorylation; muscle cell differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of osteoblast proliferation; negative regulation of ubiquitin-protein ligase activity; autophagy; response to oxidative stress; actin cytoskeleton organization and biogenesis; axon guidance; peptidyl-tyrosine phosphorylation; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; mitochondrial depolarization; positive regulation of mitotic cell cycle; Bergmann glial cell differentiation; regulation of transcription, DNA-dependent; collateral sprouting; cell differentiation; B cell proliferation during immune response; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; cell migration; mismatch repair; thymus development; DNA damage response, signal transduction; B-1 B cell homeostasis; activated T cell proliferation; protein modification process; regulation of cell proliferation; microspike biogenesis; innate immune response; positive regulation of muscle cell differentiation; regulation of autophagy; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of cell-cell adhesion; blood coagulation; response to DNA damage stimulus

Disease: Leukemia, Chronic Myeloid