APLP1 elisa kit

Human APLP1 ELISA Kit

Cat. Num. AAA1752881

• Gene Names: APLP1; APLP
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: APLP1; Human APLP1 ELISA Kit; Human APLP1; Amyloid-like protein 1; APLP; APLP-1; C30; APLP1 elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human APLP1
• Sequence Length: 650

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APLP1 elisa kit

Principle of the Assay: The Picokine™ Human APLP1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human APLP1 with a 96-well strip plate that is pre-coated with antibody specific for APLP1. The detection antibody is a biotinylated antibody specific for APLP1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated monoclonal antibody from goat. The kit contains recombinant Human APLP1 with immunogen: Expression system for standard: NSO, Immunogen sequence: S39-E580. The kit is analytically validated with ready to use reagents. To measure Human APLP1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human APLP1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human APLP1 in the sample.!!Background/Introduction: This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 28558769
• NCBI GeneID: 333
• UniProt Accession #: P51693
• NCBI Official Full Name: Amyloid-like protein 1
• NCBI Official Synonym Full Names: amyloid beta precursor like protein 1
• NCBI Official Symbol: APLP1
• NCBI Official Synonym Symbols: APLP
• NCBI Protein Information: amyloid-like protein 1
• UniProt Protein Name: Amyloid-like protein 1
• Protein Family: Amyloid-like protein
• UniProt Gene Name: APLP1
• UniProt Synonym Gene Names: APLP; APLP-1
• UniProt Entry Name: APLP1_HUMAN

NCBI Description

This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

APLP1: May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. Belongs to the APP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to membrane; plasma membrane; basement membrane

Molecular Function: heparin binding; identical protein binding; protein binding; alpha-2C adrenergic receptor binding; transition metal ion binding; alpha-2B adrenergic receptor binding; alpha-2A adrenergic receptor binding

Biological Process: regulation of translation; nervous system development; negative regulation of cAMP biosynthetic process; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; forebrain development; mRNA polyadenylation; endocytosis; G-protein signaling, adenylate cyclase inhibiting pathway; cell adhesion