Rabbit anti-Human CD82 Polyclonal Antibody | anti-CD82 antibody

Anti-CD82 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106351

• Gene Names: CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CD82; Polyclonal Antibody; Anti-CD82 Antibody; Rabbit Polyclonal; CD82/KAI-1 Antibody; Rabbit PAb; Antigen Affinity Purified; CD82 molecule; Anti-4F9 Antibody; Anti-C33 Antibody; Anti-GR15 Antibody; Anti-IA4 Antibody; Anti-KAI-1 Antibody; Anti-KAI1 Antibody; Anti-R2 Antibody; Anti-SAR2 Antibody; Anti-ST6 Antibody; Anti-TSPAN27 Antibody; anti-CD82 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD82/KAI-1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD82 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human CD82/KAI-1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD82/KAI-1 (rh CD82/KAI-1). CD82/KAI-1 specific IgG was purified by Human CD82/KAI-1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CD82 in human endometrium with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelium in uterine-gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunoprecipitation (IP)

(CD82 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-CD82 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD82 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 36 kDa)

Western Blot (WB)

(Anti-CD82 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:32 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CD82 antibody

CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.

References

Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.

NCBI and Uniprot Product Information

• NCBI GI #: 4504813
• NCBI GeneID: 3732
• NCBI Accession #: NP_002222
• NCBI GenBank Nucleotide #: NM_002231.3
• UniProt Accession #: P27701; Q7Z2D4; Q7Z5N2; D3DQN6; E9PC70
• Molecular Weight: 26,818 Da
• NCBI Official Full Name: CD82 antigen isoform 1
• NCBI Official Synonym Full Names: CD82 molecule
• NCBI Official Symbol: CD82
• NCBI Official Synonym Symbols: R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• NCBI Protein Information: CD82 antigen; tspan-27; C33 antigen; tetraspanin-27; inducible membrane protein R2; metastasis suppressor Kangai-1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
• UniProt Protein Name: CD82 antigen
• Protein Family: CD82 antigen
• UniProt Gene Name: CD82
• UniProt Synonym Gene Names: KAI1; SAR2; ST6; TSPAN27; Tspan-27
• UniProt Entry Name: CD82_HUMAN

NCBI Description

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD82: a widespread transmembrane protein of the tetraspanin family. A metastasis-suppressor whose decreased expression may be involved in malignant progression. Suppresses tumor metastasis of many cancers including cancers of the prostate, bladder, colon, cervix, liver, and lung (NSCLC). It is a target of estrogen receptor-mediated gene repression and is downregulated in primary human breast cancer. May be a prognostic marker for lung cancer and tumor metastatic potential. Interacts with cell surface proteins including integrins, cadherins, CD4, CD8, IGSF8. Modulates EGFR signaling. May suppress invasion by inhibiting integrin-dependent crosstalk with c-Met receptor and Src kinases. Its regulation of c-Met signaling apparently affects cancer cell migration.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Disease: Prostate Cancer