Typical Testing Data/Standard Curve (for reference only)
Human ANGPTL8 ELISA Kit | ANGPTL8 elisa kit
Human ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit
Gene Names
C19orf80; RIFL; TD26; ANGPTL8; PRO1185; PVPA599
Reactivity
Human
Synonyms
ANGPTL8; Human ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit; ANGPTL8 elisa kit
Reactivity
Human
Specificity
This kit recognizes Human ANGPTL8 in samples. No significant cross-reactivity or interference between Human ANGPTL8 and analogues was observed.
Sequence Length
198
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
125-8000pg/mL
Sensitivity
75pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ANGPTL8 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ANGPTL8 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ANGPTL8 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ANGPTL8 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ANGPTL8. You can calculate the concentration of Human ANGPTL8 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ANGPTL8. You can calculate the concentration of Human ANGPTL8 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,105 Da
NCBI Official Full Name
angiopoietin-like protein 8
NCBI Official Synonym Full Names
chromosome 19 open reading frame 80
NCBI Official Symbol
C19orf80
NCBI Official Synonym Symbols
RIFL; TD26; ANGPTL8; PRO1185; PVPA599
NCBI Protein Information
angiopoietin-like protein 8; betatrophin variant 1; betatrophin variant 2; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; lipasin; refeeding-induced fat and liver protein
UniProt Protein Name
Angiopoietin-like protein 8
Protein Family
UniProt Gene Name
C19orf80
UniProt Entry Name
BETAT_HUMAN
Uniprot Description
EG624219: Hormone that specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance. Promotes pancreatic beta cell proliferation without insulin resistance. Also acts as a blood lipid regulator by regulating serum triglyceride levels; possibly by promoting ANGPTL3 cleavage. In response to food intake. Interacts with ANGPTL3
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extracellular region
Molecular Function: protein binding; hormone activity
Biological Process: fat cell differentiation; regulation of lipid metabolic process; cell maturation; regulation of lipoprotein metabolic process; cellular lipid metabolic process
Research Articles on ANGPTL8
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Product Notes
The Human ANGPTL8 c19orf80 (Catalog #AAA2515814) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515814 ELISA Kit recognizes Human ANGPTL8. It is sometimes possible for the material contained within the vial of "ANGPTL8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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