Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) RTU ELISA Kit | PPARgC1a rtu elisa kit

Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit

Cat. Num. AAA4501977

• Gene Names: PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
• Reactivity: Human
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a); Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit; LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPARGC1; Ligand effect modulator 6; PPARgC1a rtu elisa kit

• Reactivity: Human
• Sequence Length: 803

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPARgC1a rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPARgC1a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PPARgC1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PPARgC1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PPARgC1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1064788214
• NCBI GeneID: 10891
• NCBI Accession #: NP_001317680.1
• NCBI GenBank Nucleotide #: NM_001330751.1
• UniProt Accession #: Q9UBK2; Q3LIG1; Q4W5M7; Q9UN32; B7Z406; G8DM16; I3RTT5; I3RTT6; I3RTT7; I3RTT8; I3RTT9
• Molecular Weight: 77,139 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma coactivator 1-alpha isoform 1
• NCBI Official Synonym Full Names: PPARG coactivator 1 alpha
• NCBI Official Symbol: PPARGC1A
• NCBI Official Synonym Symbols: LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• Protein Family: Peroxisome proliferator-activated receptor gamma coactivator
• UniProt Gene Name: PPARGC1A
• UniProt Synonym Gene Names: LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha

NCBI Description

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.