Human Angiogenin/ANG ELISA Kit | ANG elisa kit

Human Angiogenin/ANG PicoKine Fast ELISA Kit

Cat. Num. AAA1752735

• Gene Names: ANG; ALS9; RAA1; HEL168; RNASE4; RNASE5
• Reactivity: Human
• Synonyms: Angiogenin/ANG; Human Angiogenin/ANG PicoKine Fast ELISA Kit; Human Angiopoietin-1; Angiogenin; 3.1.27.-; Ribonuclease 5; RNase 5; ANG; RNASE5; ANG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human ANG
• Sequence Length: 147

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: <12pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG elisa kit

Principle of the Assay: The Picokine™ Human ANG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ANG with a 96-well strip plate that is pre-coated with antibody specific for ANG. The detection antibody is a biotinylated antibody specific for ANG. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ANG with immunogen: Expression system for standard: E.coli; Immunogen sequence: Q25-P147. The kit is analytically validated with ready to use reagents. To measure Human ANG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human ANG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANG in the sample.!!Background/Introduction: Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

NCBI and Uniprot Product Information

• NCBI GI #: 4557313
• NCBI GeneID: 283
• NCBI Accession #: NP_001136.1
• NCBI GenBank Nucleotide #: NP_001136.1
• UniProt Accession #: P03950
• NCBI Official Full Name: angiogenin
• NCBI Official Synonym Full Names: angiogenin
• NCBI Official Symbol: ANG
• NCBI Official Synonym Symbols: ALS9; RAA1; HEL168; RNASE4; RNASE5
• NCBI Protein Information: angiogenin
• UniProt Protein Name: Angiogenin
• Protein Family: Angiogenin
• UniProt Gene Name: ANG
• UniProt Synonym Gene Names: RNASE5; RNase 5
• UniProt Entry Name: ANGI_HUMAN

NCBI Description

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]

Uniprot Description

ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Ribonuclease; Secreted; Extracellular matrix; Cell cycle regulation; EC 3.1.27.-; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 14q11.1-q11.2

Cellular Component: extracellular space; angiogenin-PRI complex; growth cone; cell soma; nucleolus; basal lamina; nucleus

Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; ribonuclease activity; receptor binding

Biological Process: cell migration; diacylglycerol biosynthetic process; negative regulation of smooth muscle cell proliferation; activation of protein kinase B; activation of phospholipase A2; response to hormone stimulus; oocyte maturation; rRNA transcription; positive regulation of protein secretion; ovarian follicle development; homeostatic process; phospholipase C activation; actin filament polymerization; negative regulation of translation; response to hypoxia; positive regulation of endothelial cell proliferation; cell communication; angiogenesis; positive regulation of phosphorylation; placenta development

Disease: Amyotrophic Lateral Sclerosis 9