Testing Data
Human Angiogenin/ANG ELISA Kit | ANG elisa kit
Human Angiogenin/ANG Quick ELISA Kit
O.D.|0.035|0.060|0.177|0.398|0.795|1.363|1.996|2.429
n|16|16|16|
Mean(pg/ml)|101|751|2946|
Standard deviation|4.44|48.06|191.49|
CV(%)|4.4%|6.4%|6.5%|
n|24|24|24
Mean(pg/ml)|98|712|2931
Standard deviation|4.41|45.57|240.34
CV(%)|4.5%|6.4%|8.2%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 101|120|127|102|113|6.08|5.4%
Sample 2: 751|691|634|721|699|42.65|6.1%
Sample 3: 2946|2796|2945|2864|2888|57.76|2%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Human ANG, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ANG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANG in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Jul 2008]
Uniprot Description
ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Ribonuclease; Secreted; Extracellular matrix; Cell cycle regulation; EC 3.1.27.-; RNA-binding; Nucleolus
Chromosomal Location of Human Ortholog: 14q11.1-q11.2
Cellular Component: extracellular space; angiogenin-PRI complex; growth cone; cell soma; nucleolus; basal lamina; nucleus
Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; ribonuclease activity; receptor binding
Biological Process: cell migration; diacylglycerol biosynthetic process; negative regulation of smooth muscle cell proliferation; activation of protein kinase B; activation of phospholipase A2; response to hormone stimulus; oocyte maturation; rRNA transcription; positive regulation of protein secretion; ovarian follicle development; homeostatic process; phospholipase C activation; actin filament polymerization; negative regulation of translation; response to hypoxia; positive regulation of endothelial cell proliferation; cell communication; angiogenesis; positive regulation of phosphorylation; placenta development
Disease: Amyotrophic Lateral Sclerosis 9
Research Articles on ANG
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Product Notes
The Human ANG ang (Catalog #AAA1753903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753903 ELISA Kit recognizes Human ANG. It is sometimes possible for the material contained within the vial of "Angiogenin/ANG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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