Human Angiogenin / ANG ELISA Kit | ANG elisa kit

ANG Human ELISA Kit

Cat. Num. AAA355287

• Gene Names: ANG; ALS9; HEL168; RNASE4; RNASE5
• Reactivity: Human
• Applications: ELISA
• Synonyms: Angiogenin / ANG; ANG Human ELISA Kit; Human Angiogenin / ANG; ANG elisa kit

• Reactivity: Human

• Applicable Applications for ANG elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of ANG in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: < 12pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ANG polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ANG polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG can be calculated.

Background: Angiogenin (ANG) also known as ribonuclease 5, is a potent stimulator of new blood vessel formation. ANG is a member of the pancreatic ribonuclease A superfamily, and RNase activity of ANG is important for its angiogenic activity. It is expressed in the neuroaxis. Like VEGF, ANG is induced by hypoxia to elicit angiogenesis and is expressed in motor neurons. Angiogenin may function as a tRNA-specific ribonuclease that binds to actin on the surface of endothelial cells, once bound, angiogenin is endocytosed and translocated to the nucleus, thereby promoting the endothelial invasiveness necessary for blood vessel formation. Angiogenin induces vascularization of normal and malignant tissues, and abolishes protein synthesis by specifically hydrolyzing cellular tRNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 4557313
• NCBI GeneID: 283
• NCBI Accession #: NP_001136.1
• NCBI GenBank Nucleotide #: NM_001145.4
• UniProt Accession #: P03950; Q05CV1; Q53X86; Q6P5T2; Q8WXE7
• Molecular Weight: 16,550 Da
• NCBI Official Full Name: angiogenin
• NCBI Official Synonym Full Names: angiogenin, ribonuclease, RNase A family, 5
• NCBI Official Symbol: ANG
• NCBI Official Synonym Symbols: ALS9; HEL168; RNASE4; RNASE5
• NCBI Protein Information: angiogenin; RNase 5; ribonuclease 5; epididymis luminal protein 168
• UniProt Protein Name: Angiogenin
• Protein Family: Angiogenin
• UniProt Gene Name: ANG
• UniProt Synonym Gene Names: RNASE5; RNase 5
• UniProt Entry Name: ANGI_HUMAN

NCBI Description

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Ref.10 Ref.14 Ref.15 Ref.16

Subunit structure: Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur. Ref.13 Ref.15 Ref.18

Subcellular location: Secreted › extracellular space › extracellular matrix › basement membrane. Nucleus › nucleolus. Note: Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA. Ref.11 Ref.14

Tissue specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. Ref.9 Ref.28

Developmental stage: Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.

Involvement in disease: Amyotrophic lateral sclerosis 9 (ALS9) [MIM:611895]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31

Sequence similarities: Belongs to the pancreatic ribonuclease family.

Caution: It is uncertain whether Met-1 or Met-3 is the initiator.

Sequence caution: The sequence AAH20704.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.