Mouse MUC5AC (Mucin 5AC/Gastric Mucin) Antibody | anti-MUC5AC antibody

MUC5AC (Mucin 5AC/Gastric Mucin)

Cat. Num. AAA4381107

• Gene Names: MUC5AC; TBM; leB; MUC5; mucin
• Reactivity: Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken; Does not react with Cow.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: MUC5AC (Mucin 5AC/Gastric Mucin); Apomucin Major Airway Glycoprotein; Mucin 5 subtype AC tracheobronchial; Mucin 5 Subtypes A And C; Mucin 5AC oligomeric mucus/gel forming; Tracheobronchial Mucin (TBM); anti-MUC5AC antibody

• Host: Mouse
• Reactivity: Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken; Does not react with Cow.
• Isotype: IgG1, kappa
• Clone Number: rMUC5AC/3779
• Specificity: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 5030

• Applicable Applications for anti-MUC5AC antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human colon or stomach (IHC).
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 11p15.5
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human colon stained with MUC5AC Recombinant Mouse Monoclonal Antibody (rMUC5AC/3779).)

SDS-Page

(SDS-PAGE Analysis Purified MUC5AC Recombinant Mouse Monoclonal Antibody (rMUC5AC/3779). Confirmation of Integrity and Purity of Antibody.)

References

1. Biochem. J. 254: 185-193 (1988). 2. Cancer Res.46: 3983-3989 (1986). 2. Int. J. Cancer 47: 304-310 (1991

NCBI and Uniprot Product Information

• NCBI GI #: 160370004
• NCBI GeneID: 4586
• UniProt Accession #: P98088
• Molecular Weight: >1,000kDa
• NCBI Official Full Name: Mucin-5AC
• NCBI Official Synonym Full Names: mucin 5AC, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5AC
• NCBI Official Synonym Symbols: TBM; leB; MUC5; mucin
• NCBI Protein Information: mucin-5AC
• UniProt Protein Name: Mucin-5AC
• Protein Family: Mucin
• UniProt Gene Name: MUC5AC
• UniProt Synonym Gene Names: MUC5; MUC-5AC; LeB; TBM
• UniProt Entry Name: MUC5A_HUMAN

Uniprot Description

MUC5AC iso1:

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi lumen; fibril

Molecular Function: extracellular matrix structural constituent

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; fibril organization and biogenesis; post-translational protein modification