Human Agouti Related Protein (AGRP) ELISA Kit | AGRP elisa kit

Human Agouti Related Protein (AGRP) ELISA Kit

Cat. Num. AAA2703145

• Gene Names: AGRP; ART; AGRT; ASIP2
• Reactivity: Human
• Synonyms: Agouti Related Protein (AGRP); Human Agouti Related Protein (AGRP) ELISA Kit; AGRT; ASIP2; ART; Agouti-Related Peptide; AGRP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Agouti Related Protein (AGRP).; No significant cross-reactivity or interference between Agouti Related Protein (AGRP) and analogues was observed.
• Sequence Length: 132

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: < 3.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Agouti Related Protein (AGRP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Agouti Related Protein (AGRP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGRP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Agouti Related Protein (AGRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Agouti Related Protein (AGRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Agouti Related Protein (AGRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Agouti Related Protein (AGRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4501995
• NCBI GeneID: 181
• NCBI Accession #: NP_001129.1
• NCBI GenBank Nucleotide #: NM_001138.1
• UniProt Accession #: O00253; O15459; Q2TBD9
• Molecular Weight: 14,440 Da
• NCBI Official Full Name: agouti-related protein
• NCBI Official Synonym Full Names: agouti related neuropeptide
• NCBI Official Symbol: AGRP
• NCBI Official Synonym Symbols: ART; AGRT; ASIP2
• NCBI Protein Information: agouti-related protein
• UniProt Protein Name: Agouti-related protein
• Protein Family: Agouti-related protein
• UniProt Gene Name: AGRP
• UniProt Synonym Gene Names: AGRT; ART

NCBI Description

This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]

Uniprot Description

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.