Rabbit PHLDA2 ELISA Kit | PHLDA2 elisa kit
Rabbit PHLDA2 (Pleckstrin Homology Like Domain Family A, Member 2) ELISA Kit
Gene Names
PHLDA2; IPL; BRW1C; BWR1C; HLDA2; TSSC3
Reactivity
Rabbit
Synonyms
PHLDA2; Rabbit PHLDA2 (Pleckstrin Homology Like Domain Family A; Member 2) ELISA Kit; PHLDA2 elisa kit
Reactivity
Rabbit
Specificity
This kit recognizes natural and recombinant Rabbit PHLDA2. No significant cross-reactivity or interference between Rabbit PHLDA2 and analogues was observed.
Sequence Length
152
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
7.813-500ng/mL
Sensitivity
Min: 4.688ng/mL; Max: 500ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PHLDA2 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit PHLDA2 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PHLDA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PHLDA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PHLDA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PHLDA2. You can calculate the concentration of PHLDA2 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PHLDA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PHLDA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PHLDA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PHLDA2. You can calculate the concentration of PHLDA2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,092 Da
NCBI Official Full Name
pleckstrin homology-like domain family A member 2
NCBI Official Synonym Full Names
pleckstrin homology-like domain, family A, member 2
NCBI Official Symbol
PHLDA2
NCBI Official Synonym Symbols
IPL; BRW1C; BWR1C; HLDA2; TSSC3
NCBI Protein Information
pleckstrin homology-like domain family A member 2; beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein; imprinted in placenta and liver protein; p17-BWR1C; p17-Beckwith-Wiedemann region 1 C; p17-Beckwith-Wiedemann region 1C; tumor suppressing subchromosomal transferable fragment cDNA 3; tumor suppressing subtransferable candidate 3; tumor-suppressing STF cDNA 3 protein; tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein; tumor-supressing STF cDNA 3
UniProt Protein Name
Pleckstrin homology-like domain family A member 2
Protein Family
UniProt Gene Name
PHLDA2
UniProt Synonym Gene Names
BWR1C; HLDA2; IPL; TSSC3; p17-BWR1C
UniProt Entry Name
PHLA2_HUMAN
NCBI Description
This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]
Uniprot Description
PHLDA2: a protein may play a role in regulating placenta growth. Contains one PH domain. Expressed in placenta and adult prostate gland. Expressed at low levels in adult liver and lung, and fetal liver. Expressed in adult brain and neuroblastoma, medullablastoma and glioblastoma cell lines.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: membrane; cytoplasm
Biological Process: organ morphogenesis; apoptosis; regulation of gene expression; regulation of embryonic development; regulation of cell migration; placenta development
