Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Afamin (AFM) Standard Curve.)
Human Afamin (AFM) ELISA Kit | AFM elisa kit
Human Afamin (AFM) ELISA Kit
Gene Names
AFM; ALF; ALB2; ALBA
Reactivity
Human
Synonyms
Afamin (AFM); Human Afamin (AFM) ELISA Kit; AFM; ALB2; ALBA; ALF; MGC125338; MGC125339; alpha-albumin; AFM elisa kit
Reactivity
Human
Specificity
Human Afamin (AFM) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFM. No significant cross-reactivity or interference between Human AFM and analogues was observed.
Sequence Length
599
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Afamin (AFM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Afamin (AFM) Standard Curve.)
Related Product Information for AFM elisa kit
Description: This Human Afamin (AFM) ELISA Kit employs a two-site sandwich ELISA to quantitate AFM in samples. An antibody specific for AFM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Afamin is a member of the albumin gene family, which comprises four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. The 'new' gene, called alpha-albumin, was located 10 kb downstream from the AFP locus. The gene is selectively expressed in the liver at late stages of development. The mRNA sequence encodes a predicted secreted protein with the typical triple domain disulfide cross-linked structure. Comparisons of coding and promoter sequences suggested that ALBA could be a phylogenetic intermediate between the ALB and AFP genes.
Background: Afamin is a member of the albumin gene family, which comprises four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. The 'new' gene, called alpha-albumin, was located 10 kb downstream from the AFP locus. The gene is selectively expressed in the liver at late stages of development. The mRNA sequence encodes a predicted secreted protein with the typical triple domain disulfide cross-linked structure. Comparisons of coding and promoter sequences suggested that ALBA could be a phylogenetic intermediate between the ALB and AFP genes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
afamin
NCBI Official Synonym Full Names
afamin
NCBI Official Symbol
AFM
NCBI Official Synonym Symbols
ALF; ALB2; ALBA
NCBI Protein Information
afamin
UniProt Protein Name
Afamin
Protein Family
UniProt Gene Name
AFM
UniProt Synonym Gene Names
ALB2; ALBA; Alpha-Alb
NCBI Description
This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
Uniprot Description
Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed:26902720). Binds vitamin E (PubMed:15952736, PubMed:12463752). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed:15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed:19046407).
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Product Notes
The Human AFM afm (Catalog #AAA9714620) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714620 ELISA Kit recognizes Human AFM. It is sometimes possible for the material contained within the vial of "Afamin (AFM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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