Western Blot (WB)
((1ug/ml) staining of MDCK lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat TTLL4 Polyclonal Antibody | anti-TTLL4 antibody
Goat anti-TTLL4 Antibody
Reactivity
Tested: Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TTLL4; Polyclonal Antibody; Goat anti-TTLL4 Antibody; Goat Anti-TTLL4 Antibody; tubulin tyrosine ligase-like family; member 4; KIAA0173; OTTHUMP00000164099; KIAA0173 antibody; OTTHUMP00000164099 antibody; member 4 antibody; TTLL4 antibody; anti-TTLL4 antibody
Host
Goat
Reactivity
Tested: Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RSSYRQKPYQQLE
Sequence Length
1199
Applicable Applications for anti-TTLL4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 150kDa band observed in lysates of cell line MDCK (calculated MW of 133kDa according to NP_055455.3). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 150kDa band observed in lysates of cell line MDCK (calculated MW of 133kDa according to NP_055455.3). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-RSSYRQKPYQQLE, from the internal region of the protein sequence according to NP_055455.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of MDCK lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((1ug/ml) staining of MDCK lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,378 Da
NCBI Official Full Name
tubulin polyglutamylase TTLL4
NCBI Official Synonym Full Names
tubulin tyrosine ligase like 4
NCBI Official Symbol
TTLL4
NCBI Protein Information
tubulin polyglutamylase TTLL4
UniProt Protein Name
Tubulin polyglutamylase TTLL4
Protein Family
UniProt Gene Name
TTLL4
UniProt Synonym Gene Names
KIAA0173
UniProt Entry Name
TTLL4_HUMAN
Uniprot Description
TTLL4: Polyglutamylase which preferentially modifies beta- tubulin and nucleosome assembly proteins NAP1 and NAP2. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step. Belongs to the tubulin--tyrosine ligase family.
Protein type: EC 6.-.-.-; Ligase
Chromosomal Location of Human Ortholog: 2p24.3-p24.1
Cellular Component: cilium; cytoplasm; microtubule
Molecular Function: ATP binding; tubulin binding
Biological Process: peptidyl-glutamic acid modification; protein polyglutamylation
Research Articles on TTLL4
Similar Products
Product Notes
The TTLL4 ttll4 (Catalog #AAA423493) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TTLL4 Antibody reacts with Tested: Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TTLL4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 150kDa band observed in lysates of cell line MDCK (calculated MW of 133kDa according to NP_055455.3). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the TTLL4 ttll4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RSSYRQKPYQ QLE. It is sometimes possible for the material contained within the vial of "TTLL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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