Human afamin ELISA Kit | AFM elisa kit

Human Afamin, AFM ELISA Kit

Cat. Num. AAA918191

• Gene Names: AFM; ALF; ALB2; ALBA
• Reactivity: Human
• Synonyms: afamin; Human Afamin; AFM ELISA Kit; Human Afamin (AFM) ELISA kit; ALB2; ALBA; ALF; MGC125338; MGC125339; alpha-albumin; AFM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human AFM. No significant cross-reactivity or interference between human AFM and analogues was observed.
• Sequence Length: 599

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 3.9 ng/ml-250 ng/ml
• Sensitivity: <0.97 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AFM elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4501987
• NCBI GeneID: 173
• NCBI Accession #: NP_001124.1
• NCBI GenBank Nucleotide #: NM_001133.2
• UniProt Accession #: P43652; Q32MR3; Q4W5C5; A8K3E1
• Molecular Weight: 69,069 Da
• NCBI Official Full Name: afamin
• NCBI Official Synonym Full Names: afamin
• NCBI Official Symbol: AFM
• NCBI Official Synonym Symbols: ALF; ALB2; ALBA
• NCBI Protein Information: afamin; alpha-Alb; alpha-albumin
• UniProt Protein Name: Afamin
• Protein Family: Afamin
• UniProt Gene Name: AFM
• UniProt Synonym Gene Names: ALB2; ALBA; Alpha-Alb
• UniProt Entry Name: AFAM_HUMAN

NCBI Description

This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]

Uniprot Description

A-albumin: Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted, signal peptide; Secreted; Carrier

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: vitamin E binding

Biological Process: vitamin transport