Human ADP ribosylation factor like protein 13B (ARL13B) ELISA Kit | ARL13B elisa kit

Human ADP ribosylation factor like protein 13B (ARL13B) ELISA Kit

Cat. Num. AAA7248405

• Gene Names: ARL13B; JBTS8; ARL2L1
• Reactivity: Human
• Synonyms: ADP ribosylation factor like protein 13B (ARL13B); Human ADP ribosylation factor like protein 13B (ARL13B) ELISA Kit; ARL13B elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for ARL13B elisa kit

Intended Uses: This ARL13B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARL13B. This ELISA kit for research use only!

Principle of the Assay||ARL13B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARL13B antibody and an ARL13B-HRP conjugate. The assay sample and buffer are incubated together with ARL13B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARL13B concentration since ARL13B from samples and ARL13B-HRP conjugate compete for the anti-ARL13B antibody binding site. Since the number of sites is limited, as more sites are occupied by ARL13B from the sample, fewer sites are left to bind ARL13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARL13B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ARL13B elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 115503786
• NCBI GeneID: 200894
• Molecular Weight: 37,087 Da
• NCBI Official Full Name: ADP-ribosylation factor-like protein 13B
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 13B
• NCBI Official Symbol: ARL13B
• NCBI Official Synonym Symbols: JBTS8; ARL2L1
• NCBI Protein Information: ADP-ribosylation factor-like protein 13B; ARL2-like protein 1; ADP-ribosylation factor-like 2-like 1
• UniProt Protein Name: ADP-ribosylation factor-like protein 13B
• UniProt Gene Name: ARL13B
• UniProt Synonym Gene Names: ARL2L1; ARL2-like protein 1
• UniProt Entry Name: AR13B_HUMAN

NCBI Description

This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

ARL13B: Defects in ARL13B are the cause of Joubert syndrome type 8 (JBTS8). JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermis hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, ARF; G protein, monomeric

Chromosomal Location of Human Ortholog: 3q11.1

Cellular Component: intracellular; cilium

Molecular Function: protein binding; GTP binding

Biological Process: formation of radial glial scaffolds; smoothened signaling pathway; dorsal/ventral pattern formation; sensory cilium biogenesis; interneuron migration from the subpallium to the cortex; organelle organization and biogenesis; small GTPase mediated signal transduction; neural tube patterning; cilium biogenesis; heart looping

Disease: Joubert Syndrome 8