Rabbit ARL13B Polyclonal Antibody | anti-ARL13B antibody

ARL13B Polyclonal Antibody

Cat. Num. AAA9126992

• Gene Names: ARL13B; JBTS8; ARL2L1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: ARL13B; Polyclonal Antibody; ARL13B Polyclonal Antibody; ARL2L1; JBTS8; ADP ribosylation factor like GTPase 13B; anti-ARL13B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 428

• Applicable Applications for anti-ARL13B antibody: Western Blot (WB)
• Application Notes: Recommended Dilutions: WB 1:500 - 1:2000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ARL13B (NP_878899.1).
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of SKOV3 cells, using ARL13B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using ARL13B antibody (MBS9126992) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U-2 OS cells using ARL13B antibody (MBS9126992) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of L929 cells using ARL13B antibody (MBS9126992) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-ARL13B antibody

This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-ARL13B antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 115503786
• NCBI GeneID: 200894
• UniProt Accession #: Q3SXY8; Q504W8; Q8TCL5; D3DN29; G3V1S8
• Molecular Weight: Observed: 49kDa; Calculated: 36kDa/37kDa/48kDa
• NCBI Official Full Name: ADP-ribosylation factor-like protein 13B
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 13B
• NCBI Official Symbol: ARL13B
• NCBI Official Synonym Symbols: JBTS8; ARL2L1
• NCBI Protein Information: ADP-ribosylation factor-like protein 13B; ARL2-like protein 1; ADP-ribosylation factor-like 2-like 1
• UniProt Protein Name: ADP-ribosylation factor-like protein 13B
• UniProt Gene Name: ARL13B
• UniProt Synonym Gene Names: ARL2L1; ARL2-like protein 1
• UniProt Entry Name: AR13B_HUMAN

NCBI Description

This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

ARL13B: Defects in ARL13B are the cause of Joubert syndrome type 8 (JBTS8). JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermis hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 3q11.1

Cellular Component: intracellular; cilium

Molecular Function: protein binding; GTP binding

Biological Process: formation of radial glial scaffolds; smoothened signaling pathway; dorsal/ventral pattern formation; interneuron migration from the subpallium to the cortex; sensory cilium biogenesis; organelle organization and biogenesis; small GTPase mediated signal transduction; neural tube patterning; cilium biogenesis; heart looping

Disease: Joubert Syndrome 8