Rabbit anti-Human PRDX1 Polyclonal Antibody | anti-PRDX1 antibody

PRDX1, ID (Peroxiredoxin-1, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, Proliferation-associated Gene Protein, PAG, Natural Killer Cell-enhancing Factor A, NKEF-A, TDPX2, PAGB, PAGA) (MaxLight 750)

Cat. Num. AAA6492711

• Gene Names: PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PRDX1; Polyclonal Antibody; ID (Peroxiredoxin-1; Thioredoxin Peroxidase 2; Thioredoxin-dependent Peroxide Reductase 2; Proliferation-associated Gene Protein; PAG; Natural Killer Cell-enhancing Factor A; NKEF-A; TDPX2; PAGB; PAGA) (MaxLight 750); anti-PRDX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRDX1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-PRDX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PRDX1 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRDX1 Antibody (Center) in CEM cell line lysates (35ug/lane). PRDX1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PRDX1 (arrow) using rabbit polyclonal PRDX1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX1 gene (Lane 2).)

Flow Cytometry (FC/FACS)

(PRDX1 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(PRDX1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRDX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-PRDX1 antibody

Antibodies; Enzymes; Peroxiredoxin

References

1. Song, I.S., et.al., Carcinogenesis 30(7), 1106-1114 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4505591
• NCBI GeneID: 5052
• NCBI Accession #: NP_002565
• NCBI GenBank Nucleotide #: NM_002574.3
• UniProt Accession #: Q06830; P35703; Q2V576; Q5T154; Q5T155; B5BU26; D3DPZ8
• Molecular Weight: 22,110 Da
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: PRDX1
• NCBI Official Synonym Symbols: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• NCBI Protein Information: peroxiredoxin-1
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX1
• UniProt Synonym Gene Names: PAGA; PAGB; TDPX2; NKEF-A; PAG
• UniProt Entry Name: PRDX1_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; EC 1.11.1.15

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: peroxisomal matrix; extracellular space; mitochondrial matrix; cytoplasm; nucleolus; melanosome; nucleus; cytosol

Molecular Function: protein binding; peroxidase activity; protein homodimerization activity; thioredoxin peroxidase activity; heme binding

Biological Process: cell proliferation; retinal homeostasis; erythrocyte homeostasis; regulation of stress-activated MAPK cascade; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; natural killer cell mediated cytotoxicity; skeletal development; regulation of NF-kappaB import into nucleus