Typical Testing Data/Standard Curve (for reference only)
Human Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) ELISA Kit | ASCC3 elisa kit
Human Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) ELISA Kit
Gene Names
Ascc3; RNAH; Helic1; ASC1p200; BC023451; D630041L21; B630009I04Rik; D430001L07Rik
Reactivity
Human
Synonyms
Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3); Human Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) ELISA Kit; HELIC1; RNAH; ASC1p200; RNA Helicase Family; Helicase; ATP Binding 1; Trip4 complex subunit p200; ASC-1 complex subunit p200; ASCC3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3).
No significant cross-reactivity or interference between Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) and analogues was observed.
No significant cross-reactivity or interference between Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) and analogues was observed.
Sequence Length
2198
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.122ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ASCC3 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128,982 Da
NCBI Official Full Name
activating signal cointegrator 1 complex subunit 3
NCBI Official Synonym Full Names
activating signal cointegrator 1 complex subunit 3
NCBI Official Symbol
Ascc3
NCBI Official Synonym Symbols
RNAH; Helic1; ASC1p200; BC023451; D630041L21; B630009I04Rik; D430001L07Rik
NCBI Protein Information
activating signal cointegrator 1 complex subunit 3
UniProt Protein Name
Activating signal cointegrator 1 complex subunit 3
Protein Family
UniProt Gene Name
Ascc3
Uniprot Description
ASCC3: 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3- methylcytosine (3mC) within double-stranded regions. Enhances NF- kappa-B, SRF and AP1 transactivation. Belongs to the helicase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.6.4.12; Helicase; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10|10 B3
Cellular Component: activating signal cointegrator 1 complex; cytosol; intracellular; membrane; nucleus
Molecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; helicase activity; hydrolase activity; nucleic acid binding; nucleotide binding; RNA binding
Biological Process: cell proliferation; cellular response to DNA damage stimulus; DNA dealkylation involved in DNA repair; DNA duplex unwinding; DNA repair; regulation of transcription, DNA-templated; transcription, DNA-dependent
Similar Products
Product Notes
The Human ASCC3 ascc3 (Catalog #AAA2707270) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707270 ELISA Kit recognizes Human ASCC3. It is sometimes possible for the material contained within the vial of "Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
