Rabbit anti-Human HEBP1 Polyclonal Antibody | anti-HEBP1 antibody

HEBP1, NT (HEBP1, HBP, Heme-binding protein 1, p22HBP) (Azide free) (HRP)

Cat. Num. AAA6306014

• Gene Names: HEBP1; HBP; HEBP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HEBP1; Polyclonal Antibody; NT (HEBP1; HBP; Heme-binding protein 1; p22HBP) (Azide free) (HRP); p22HBP; anti-HEBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HEBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HEBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human HEBP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HEBP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HEBP1 antibody detected the HEBP1 protein (arrow).)

Related Product Information for anti-HEBP1 antibody

The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells. [provided by RefSeq].

Product Categories/Family for anti-HEBP1 antibody

Antibodies; Binding Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Lambert, N.A. Sci Signal 1 (25), RE5 (2008): Gao, J.L., et al. J. Immunol. 178(3):1450-1456(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 20336761
• NCBI GeneID: 50865
• NCBI Accession #: NP_057071.2
• NCBI GenBank Nucleotide #: NM_015987.4
• UniProt Accession #: Q9NRV9; Q9Y5Z5; A8K1G2
• Molecular Weight: 21,097 Da
• NCBI Official Full Name: heme-binding protein 1
• NCBI Official Synonym Full Names: heme binding protein 1
• NCBI Official Symbol: HEBP1
• NCBI Official Synonym Symbols: HBP; HEBP
• NCBI Protein Information: heme-binding protein 1; p22HBP
• UniProt Protein Name: Heme-binding protein 1
• Protein Family: Heme-binding protein
• UniProt Gene Name: HEBP1
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: HEBP1_HUMAN

NCBI Description

The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells. [provided by RefSeq, Jul 2008]

Uniprot Description

HEBP1: May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N- methylprotoporphyrin with similar affinities. Belongs to the HEBP family.

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: mitochondrion; extracellular region; cytosol

Molecular Function: heme binding

Biological Process: circadian rhythm