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Typical Testing Data/Standard Curve (for reference only)

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit | ALCAM elisa kit

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

Gene Names
ALCAM; MEMD; CD166
Reactivity
Human
Synonyms
Activated Leukocyte Cell Adhesion Molecule (ALCAM); Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit; CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule; ALCAM elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM).
No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.
Sequence Length
305
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.068ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ALCAM elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activated Leukocyte Cell Adhesion Molecule (ALCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activated Leukocyte Cell Adhesion Molecule (ALCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ALCAM elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
214
UniProt Accession #
Molecular Weight
33,878 Da
NCBI Official Full Name
Activated leukocyte cell adhesion molecule
NCBI Official Synonym Full Names
activated leukocyte cell adhesion molecule
NCBI Official Symbol
ALCAM
NCBI Official Synonym Symbols
MEMD; CD166
NCBI Protein Information
CD166 antigen
UniProt Protein Name
CD166 antigen
Protein Family
UniProt Gene Name
ALCAM
UniProt Synonym Gene Names
MEMD
UniProt Entry Name
CD166_HUMAN

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; cell adhesion; signal transduction

Research Articles on ALCAM

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Product Notes

The Human ALCAM alcam (Catalog #AAA2024239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024239 ELISA Kit recognizes Human ALCAM. It is sometimes possible for the material contained within the vial of "Activated Leukocyte Cell Adhesion Molecule (ALCAM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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