Human Abl Interactor 1 (ABI1) ELISA Kit | ABI1 elisa kit

Human Abl Interactor 1 (ABI1) ELISA Kit

Cat. Num. AAA450463

• Gene Names: ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
• Reactivity: Human
• Synonyms: Abl Interactor 1 (ABI1); Human Abl Interactor 1 (ABI1) ELISA Kit; ABI-1; E3B1; NAP1BP; SSH3BP1; Spectrin SH3 Domain Binding Protein 1; Abelson interactor 1; Abl-binding protein 4; Nap1-binding protein; Eps8 SH3 domain-binding protein; ABI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ABI1. No significant cross-reactivity or interference between ABI1 and analogues was observed.
• Sequence Length: 481

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ABI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ABI1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABI1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ABI1 in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABI1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ABI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ABI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ABI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 61743944
• NCBI GeneID: 10006
• NCBI Accession #: NP_001012768.1
• NCBI GenBank Nucleotide #: NM_001012750.2
• UniProt Accession #: Q8IZP0; O15147; O76049; O95060; Q5T2R3; Q5T2R4; Q5T2R6; Q5T2R7; A9Z1Y6; B3KX62; B4DQ58; H7BXI6
• Molecular Weight: 53,912 Da
• NCBI Official Full Name: abl interactor 1 isoform b
• NCBI Official Synonym Full Names: abl interactor 1
• NCBI Official Symbol: ABI1
• NCBI Official Synonym Symbols: E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
• NCBI Protein Information: abl interactor 1
• UniProt Protein Name: Abl interactor 1
• Protein Family: Abl interactor
• UniProt Gene Name: ABI1
• UniProt Synonym Gene Names: SSH3BP1; Abi-1; AblBP4; Eps8-binding protein; Nap1BP

NCBI Description

This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Sep 2011]

Uniprot Description

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.