Rabbit CYP7B1 Polyclonal Antibody | anti-CYP7B1 antibody

CYP7B1 (Q127) Polyclonal Antibody

Cat. Num. AAA3003324

• Gene Names: CYP7B1; CP7B; CBAS3; SPG5A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CYP7B1; Polyclonal Antibody; CYP7B1 (Q127) Polyclonal Antibody; 25-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase; anti-CYP7B1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CYP7B1 (Q127) polyclonal antibody detects endogenous levels of CYP7B1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 506

• Applicable Applications for anti-CYP7B1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human CYP7B1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CYP7B1 (Q127) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing strong membranous and cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CYP7B1 (Q127) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing strong membranous and cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CYP7B1 (Q127) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing strong membranous and cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-CYP7B1 antibody

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR.CYP7B1 (oxysterol 7-alpha-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25- and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.

NCBI and Uniprot Product Information

• NCBI GI #: 4758104
• NCBI GeneID: 9420
• NCBI Accession #: NP_004811.1
• NCBI GenBank Nucleotide #: NP_004811.1
• UniProt Accession #: O75881
• Molecular Weight: ~ 58kDa
• NCBI Official Full Name: cytochrome P450 7B1 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 7 subfamily B member 1
• NCBI Official Symbol: CYP7B1
• NCBI Official Synonym Symbols: CP7B; CBAS3; SPG5A
• NCBI Protein Information: cytochrome P450 7B1
• UniProt Protein Name: 25-hydroxycholesterol 7-alpha-hydroxylase
• Protein Family: 25-hydroxycholesterol 7-alpha-hydroxylase
• UniProt Gene Name: CYP7B1
• UniProt Entry Name: CP7B1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]

Uniprot Description

CYP7B1: Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3). Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; EC 1.14.13.100; Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: endoplasmic reticulum membrane

Molecular Function: 25-hydroxycholesterol 7alpha-hydroxylase activity; iron ion binding; heme binding; oxysterol 7-alpha-hydroxylase activity

Biological Process: cholesterol metabolic process; bile acid biosynthetic process; bile acid metabolic process; xenobiotic metabolic process; sterol metabolic process; transmembrane transport; positive regulation of epithelial cell proliferation; negative regulation of estrogen receptor signaling pathway

Disease: Spastic Paraplegia 5a, Autosomal Recessive; Bile Acid Synthesis Defect, Congenital, 3