Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit | ADAM15 elisa kit

Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit

Cat. Num. AAA2706771

• Reactivity: Human
• Synonyms: A Disintegrin And Metalloprotease 15 (ADAM15); Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit; MDC15; Metargidin; Metalloproteinase-like; disintegrin-like; and cysteine-rich protein 15; Metalloprotease RGD disintegrin protein; ADAM15 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 15 (ADAM15).; No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 15 (ADAM15) and analogues was observed.
• Sequence Length: 950

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 15 (ADAM15) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 15 (ADAM15) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM15 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 15 (ADAM15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 15 (ADAM15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 15 (ADAM15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 15 (ADAM15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21265058
• NCBI GeneID: 170689
• NCBI Accession #: NP_620686.1
• NCBI GenBank Nucleotide #: NM_139055.3
• UniProt Accession #: Q8TE58; Q32MI6
• Molecular Weight: 103,287 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 15 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif 15
• NCBI Official Symbol: ADAMTS15
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 15
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 15
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAMTS15
• UniProt Synonym Gene Names: ADAM-TS 15; ADAM-TS15; ADAMTS-15

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme, which may play a role in versican processing during skeletal muscle development. This gene may function as a tumor suppressor in colorectal and breast cancers. [provided by RefSeq, May 2016]

Uniprot Description

ADAMTS15:

Protein type: EC 3.4.24.-; Extracellular matrix; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q24.3

Cellular Component: cell surface; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix binding; heparin binding; metalloendopeptidase activity; zinc ion binding

Biological Process: proteolysis