Rabbit EHMT2 Antibody | anti-EHMT2 antibody

EHMT2 Antibody

Cat. Num. AAA8504063

• Gene Names: EHMT2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: EHMT2; EHMT2 Antibody; BAT8; C6orf30; DKFZp686H08213; FLJ35547; G9A; KMT1C; NG36; NG36/G9a; anti-EHMT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1233

• Applicable Applications for anti-EHMT2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide of human EHMT2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of Jurkat cellline, using EHMT2antibody.)

Related Product Information for anti-EHMT2 antibody

G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di-and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1, 2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono-and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3, 4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12, 13).

Product Categories/Family for anti-EHMT2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Tachibana, M. et al. (2001) J Biol Chem 276, 25309-17. 2. Patnaik, D. et al. (2004) J Biol Chem 279, 53248-58. 3. Tachibana, M. et al. (2002) Genes Dev 16, 1779-91. 4. Tachibana, M. et al. (2005) Genes Dev 19, 815-26. 5. Sampath, S.C. et al. (2007) Mol Cell 27, 596-608. 6. Ueda, J. et al. (2006) J Biol Chem 281, 20120-8. 7. Ogawa, H. et al. (2002) Science 296, 1132-6. 8. Shi, Y. et al. (2003) Nature 422, 735-8. 9. Nishio, H. and Walsh, M.J. (2004) Proc Natl Acad Sci USA 101, 11257-62. 10. Estève, P.O. et al. (2006) Genes Dev 20, 3089-103. 11. Tachibana, M. et al. (2007) EMBO J 26, 3346-59. 12. Kondo, Y. et al. (2007) Hepatol Res 37, 974-83.

NCBI and Uniprot Product Information

• NCBI GI #: 574957264
• NCBI GeneID: 10919
• NCBI Accession #: NP_001276342.1
• NCBI GenBank Nucleotide #: NM_001289413.1
• UniProt Accession #: Q96KQ7; Q14349; Q5JP83; Q5JQ92; Q5JQA1; Q5JQG3; Q6PK06; Q96MH5; Q96QD0; Q9UQL8; Q9Y331; B0UZY2
• Molecular Weight: 132kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase EHMT2 isoform c
• NCBI Official Synonym Full Names: euchromatic histone lysine methyltransferase 2
• NCBI Official Symbol: EHMT2
• NCBI Official Synonym Symbols: G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
• NCBI Protein Information: histone-lysine N-methyltransferase EHMT2
• UniProt Protein Name: Histone-lysine N-methyltransferase EHMT2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: EHMT2
• UniProt Synonym Gene Names: BAT8; C6orf30; G9A; KMT1C; NG36; H3-K9-HMTase 3

NCBI Description

This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.