Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) Standard Curve.)
Human 182 kDa Tankyrase-1-Binding Protein (TNKS1BP1) ELISA Kit | TNKS1BP1 elisa kit
Human 182 kDa Tankyrase-1-Binding Protein (TNKS1BP1) ELISA Kit
Gene Names
TNKS1BP1; TAB182
Reactivity
Human
Synonyms
182 kDa Tankyrase-1-Binding Protein (TNKS1BP1); Human 182 kDa Tankyrase-1-Binding Protein (TNKS1BP1) ELISA Kit; TNKS1BP1; FLJ45975; KIAA1741; TAB182; tankyrase 1-binding protein 1; tankyrase 1-binding protein of 182 kDa; TNKS1BP1 elisa kit
Reactivity
Human
Specificity
Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNKS1BP1. No significant cross-reactivity or interference between Human TNKS1BP1 and analogues was observed.
Sequence Length
1729
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) Standard Curve.)
Related Product Information for TNKS1BP1 elisa kit
Description: This Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS1BP1 in samples. An antibody specific for TNKS1BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNKS1BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS1BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS1BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNKS1BP1, has a calculated molecular mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large internal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northern blot analysis detected transcripts of 7, 4.4, and 2.3 kb in multiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 human cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized population of HeLa cells, TAB182 associated with chromosomes throughout mitosis.
Background: TNKS1BP1, has a calculated molecular mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large internal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northern blot analysis detected transcripts of 7, 4.4, and 2.3 kb in multiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 human cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized population of HeLa cells, TAB182 associated with chromosomes throughout mitosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
182 kDa tankyrase-1-binding protein
NCBI Official Synonym Full Names
tankyrase 1 binding protein 1
NCBI Official Symbol
TNKS1BP1
NCBI Official Synonym Symbols
TAB182
NCBI Protein Information
182 kDa tankyrase-1-binding protein
UniProt Protein Name
182 kDa tankyrase-1-binding protein
Protein Family
UniProt Gene Name
TNKS1BP1
UniProt Synonym Gene Names
KIAA1741; TAB182
UniProt Entry Name
TB182_HUMAN
Uniprot Description
TNKS1BP1: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Actin-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 11q12.1
Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; nuclear telomeric heterochromatin; plasma membrane; cytosol; nucleus; CCR4-NOT complex
Molecular Function: enzyme binding; ankyrin binding
Biological Process: poly(A) tail shortening; gene expression; telomere maintenance via telomerase; mRNA catabolic process, deadenylation-dependent decay
Research Articles on TNKS1BP1
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Product Notes
The Human TNKS1BP1 tnks1bp1 (Catalog #AAA9713862) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713862 ELISA Kit recognizes Human TNKS1BP1. It is sometimes possible for the material contained within the vial of "182 kDa Tankyrase-1-Binding Protein (TNKS1BP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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