Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Human Allograft Inflammatory Factor 1 (AIF1) DIY ELISA Kit | AIF1 diy elisa kit

Human Allograft Inflammatory Factor 1 (AIF1) ELISA Kit DIY Materials

Gene Names
Aif1; G1; Iba1; AIF-1; AI607846; D17H6S50E
Reactivity
Human
Synonyms
Allograft Inflammatory Factor 1 (AIF1); Human Allograft Inflammatory Factor 1 (AIF1) ELISA Kit DIY Materials; IBA1; IRT1; Daintain; Protein G1; Ionized calcium-binding adapter molecule 1; AIF1 diy elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Allograft Inflammatory Factor 1 (AIF1). No significant cross-reactivity or interference between Allograft Inflammatory Factor 1 (AIF1) and analogues was observed.
Sequence Length
147
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
31.2-2,000pg/mL
Sensitivity
13.7pg/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for AIF1 diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,911 Da
NCBI Official Full Name
allograft inflammatory factor 1
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
Aif1
NCBI Official Synonym Symbols
G1; Iba1; AIF-1; AI607846; D17H6S50E
NCBI Protein Information
allograft inflammatory factor 1
UniProt Protein Name
Allograft inflammatory factor 1
Protein Family
UniProt Gene Name
Aif1
UniProt Synonym Gene Names
Iba1; AIF-1

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17 B1|17 18.59 cM

Cellular Component: actin filament; cell projection; cytoplasm; cytosol; lamellipodium; nucleus; perikaryon; perinuclear region of cytoplasm; phagocytic cup; ruffle

Molecular Function: actin filament binding; calcium ion binding; protein binding

Biological Process: actin filament bundle formation; actin filament polymerization; negative regulation of apoptosis; negative regulation of smooth muscle cell proliferation; phagocytosis, engulfment; positive regulation of cell migration; positive regulation of muscle hyperplasia; positive regulation of nitric oxide biosynthetic process; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; Rac protein signal transduction

Research Articles on AIF1

Similar Products

Product Notes

The Human AIF1 aif1 (Catalog #AAA2089264) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089264 DIY ELISA Kit recognizes Human AIF1. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the AIF1 aif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Allograft Inflammatory Factor 1 (AIF1), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.