Typical Testing Data/Standard Curve (for reference only)
Human TH (Tyrosine Hydroxylase) CLIA Kit | TH clia kit
Human TH (Tyrosine Hydroxylase) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TH. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TH. You can calculate the concentration of TH in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.
Protein type: EC 1.14.16.2; Vesicle; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus
Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity
Biological Process: heart morphogenesis; response to peptide hormone stimulus; synaptic transmission, dopaminergic; heart development; response to lipopolysaccharide; locomotory behavior; dopamine biosynthetic process; fatty acid metabolic process; phytoalexin metabolic process; response to estradiol stimulus; response to salt stress; regulation of heart contraction; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; sensory perception of sound; visual perception; response to electrical stimulus; epinephrine biosynthetic process; glycoside metabolic process; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to pyrethroid; response to nutrient levels; response to light stimulus; anatomical structure morphogenesis; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; phthalate metabolic process; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; social behavior; learning; response to ether; synaptic vesicle amine transport; memory; organ morphogenesis; eye photoreceptor cell development; circadian sleep/wake cycle; response to ethanol; pigmentation; response to zinc ion; response to hypoxia; cerebral cortex development; response to activity; embryonic camera-type eye morphogenesis; response to water deprivation
Disease: Segawa Syndrome, Autosomal Recessive
Research Articles on TH
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Product Notes
The Human TH th (Catalog #AAA2533187) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533187 CLIA Kit recognizes Human TH. It is sometimes possible for the material contained within the vial of "TH (Tyrosine Hydroxylase), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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