Mouse Erythropoietin (EPO) ELISA Kit | EPO elisa kit

Mouse Erythropoietin (EPO) ELISA Kit

Cat. Num. AAA9718102

• Reactivity: Mouse
• Synonyms: Erythropoietin (EPO); Mouse Erythropoietin (EPO) ELISA Kit; EPO; EP; MGC138142; MVCD2; epoetin; EPO elisa kit

• Reactivity: Mouse
• Specificity: Mouse Erythropoietin (EPO) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EPO. No significant cross-reactivity or interference between Mouse EPO and analogues was observed.
• Sequence Length: 192

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Erythropoietin (EPO) Standard Curve.)

Related Product Information for EPO elisa kit

Description: This Mouse Erythropoietin (EPO) ELISA Kit employs a two-site sandwich ELISA to quantitate EPO in samples. An antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Erythropoietin is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow.Also called hematopoietin or hemopoietin, it is produced by the peritubular capillary endothelial cells in the kidney, and is the hormone that regulates red blood cell production.It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury.EPO is also involved in the wound healing process.When exogenous EPO is used as a performance-enhancing drug, it is classified as an erythropoiesis-stimulating agent (ESA). Exogenous EPO can often be detected in blood, due to slight difference from the endogenous protein, for example in features of posttranslational modification.

NCBI and Uniprot Product Information

• NCBI GI #: 119528
• NCBI GeneID: 13856
• UniProt Accession #: P07321
• NCBI Official Full Name: Erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_MOUSE

NCBI Description

This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; heart morphogenesis; erythrocyte maturation; apoptosis; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; negative regulation of myeloid cell apoptosis; peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of protein kinase activity; positive regulation of cell proliferation; cardiac muscle morphogensis; response to hypoxia; erythrocyte differentiation; ventricular cardiac muscle morphogenesis; angiogenesis; positive regulation of Ras protein signal transduction; positive regulation of neuron differentiation; embryo implantation; positive regulation of DNA replication; negative regulation of apoptosis