Mouse anti-Human, Mouse CSK Monoclonal Antibody | anti-CSK antibody

Anti-CSK Purified

Cat. Num. AAA1800529

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CSK; Monoclonal Antibody; Anti-CSK Purified; C-terminal Src kinase; anti-CSK antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CSK-04
• Specificity: The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Sequence Length: 450

• Applicable Applications for anti-CSK antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Immunogen: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
• Preparation and Storage: Store at 2-8 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).)

Related Product Information for anti-CSK antibody

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NP_001120662.1
• UniProt Accession #: P41240
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: C-terminal Src kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

NCBI Description

The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; negative regulation of kinase activity; negative regulation of phagocytosis; cell migration; central nervous system development; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cytokine production; regulation of cell proliferation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; T cell costimulation; negative regulation of interleukin-6 production; innate immune response; oligodendrocyte differentiation; morphogenesis of an epithelium; brain development; negative regulation of bone resorption; cell differentiation; blood coagulation; transmembrane receptor protein tyrosine kinase signaling pathway