Typical Testing Data/Standard Curve (for reference only)
Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit | Smad1/MADH1 clia kit
Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Smad1/MADH1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Smad1/MADH1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Smad1/MADH1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Smad1/MADH1. You can calculate the concentration of Smad1/MADH1 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
SMAD1: transcription factor phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated forms dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 4q31
Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; nuclear inner membrane; intracellular; cytosol; nucleus
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; receptor signaling protein activity; protein kinase binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
Biological Process: transcription from RNA polymerase II promoter; MAPKKK cascade; cardiac muscle cell proliferation; primary microRNA processing; embryonic pattern specification; signal transduction; SMAD protein complex assembly; mesodermal cell fate commitment; BMP signaling pathway; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; homeostatic process; ureteric bud development; gamete generation; transforming growth factor beta receptor signaling pathway; cartilage development; midbrain development; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; hindbrain development; osteoblast fate commitment
Research Articles on Smad1/MADH1
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Product Notes
The Human Smad1/MADH1 smad1 (Catalog #AAA2530383) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530383 CLIA Kit recognizes Human Smad1/MADH1. It is sometimes possible for the material contained within the vial of "Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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