Rabbit anti-Human Glyoxalase I Polyclonal Antibody | anti-GLO1 antibody

Glyoxalase I, NT (GLO1, GLOD1, Glx I, GLYI, Aldoketomutase, Ketone-aldehyde Mutase, Lactoylglutathione Lyase, Methylglyoxalase, S-D-lactoylglutathione Methylglyoxal Lyase) (Biotin)

Cat. Num. AAA6478563

• Gene Names: GLO1; GLYI; GLOD1; HEL-S-74
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Glyoxalase I; Polyclonal Antibody; NT (GLO1; GLOD1; Glx I; GLYI; Aldoketomutase; Ketone-aldehyde Mutase; Lactoylglutathione Lyase; Methylglyoxalase; S-D-lactoylglutathione Methylglyoxal Lyase) (Biotin); EC=4.4.1.5; anti-GLO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GLO1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GLO1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GLO1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of widr cells using GLO1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.)

Western Blot (WB)

(Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.)

Product Categories/Family for anti-GLO1 antibody

Antibodies; Enzymes; Glyoxalase

References

1. Germanova, A., Cancer Invest. 27(6), 655-660 (2009). 2. Engelen, L., J. Hypertens. 27(7), 1399-1403 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 118402586
• NCBI GeneID: 2739
• NCBI Accession #: NP_006699
• NCBI GenBank Nucleotide #: NM_006708.2
• UniProt Accession #: Q04760; P78375; Q59EL0; Q5TZW3; Q96FC0; Q96J41; B2R6P7; B4DDV0
• Molecular Weight: 20.7 kDa (184 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: lactoylglutathione lyase
• NCBI Official Synonym Full Names: glyoxalase I
• NCBI Official Symbol: GLO1
• NCBI Official Synonym Symbols: GLYI; GLOD1; HEL-S-74
• NCBI Protein Information: lactoylglutathione lyase
• UniProt Protein Name: Lactoylglutathione lyase
• UniProt Gene Name: GLO1
• UniProt Synonym Gene Names: Glx I
• UniProt Entry Name: LGUL_HUMAN

NCBI Description

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Uniprot Description

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - pyruvate; EC 4.4.1.5; Lyase

Chromosomal Location of Human Ortholog: 6p21.3-p21.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; lactoylglutathione lyase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; methylglyoxal metabolic process; negative regulation of apoptosis

Disease: Autism