Typical Testing Data/Standard Curve (for reference only)
Human KERA (Keratocan) CLIA Kit | KERA clia kit
Human KERA (Keratocan) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to KERA. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KERA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KERA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of KERA. You can calculate the concentration of KERA in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).[provided by RefSeq, May 2010]
Uniprot Description
KERA: May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix. Defects in KERA are the cause of the autosomal recessive cornea plana 2 (CNA2). In CNA2, the forward convex curvature is flattened, leading to a decrease in refraction, reduced visual activity, extreme hyperopia (usually plus 10 d or more), hazy corneal limbus, opacities in the corneal parenchyma, and marked arcus senilis (often detected at an early age). CNA2 is a rare disorder with a worldwide distribution, but a high prevalence in the Finnish population. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region
Biological Process: keratan sulfate metabolic process; glycosaminoglycan metabolic process; visual perception; response to stimulus; carbohydrate metabolic process; keratan sulfate biosynthetic process; keratan sulfate catabolic process
Disease: Cornea Plana 2
Research Articles on KERA
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Product Notes
The Human KERA kera (Catalog #AAA2531896) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531896 CLIA Kit recognizes Human KERA. It is sometimes possible for the material contained within the vial of "KERA (Keratocan), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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