Human Sestrin 1 (SESN1) ELISA Kit | SESN1 elisa kit

Human Sestrin 1 (SESN1) ELISA Kit

Cat. Num. AAA2705943

• Gene Names: SESN1; PA26; SEST1
• Reactivity: Human
• Synonyms: Sestrin 1 (SESN1); Human Sestrin 1 (SESN1) ELISA Kit; SEST1; PA26; p53-regulated protein PA26; SESN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sestrin 1 (SESN1).; No significant cross-reactivity or interference between Sestrin 1 (SESN1) and analogues was observed.
• Sequence Length: 551

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sestrin 1 (SESN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sestrin 1 (SESN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SESN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sestrin 1 (SESN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sestrin 1 (SESN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sestrin 1 (SESN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sestrin 1 (SESN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 109731079
• NCBI GeneID: 27244
• Molecular Weight: 49,624 Da
• NCBI Official Full Name: Sestrin 1
• NCBI Official Synonym Full Names: sestrin 1
• NCBI Official Symbol: SESN1
• NCBI Official Synonym Symbols: PA26; SEST1
• NCBI Protein Information: sestrin-1
• UniProt Protein Name: Sestrin-1
• Protein Family: Sestrin
• UniProt Gene Name: SESN1

NCBI Description

This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

SESN1: Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. Belongs to the sestrin family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; cytosol; fibrillar center; nucleus

Molecular Function: peroxiredoxin activity; protein binding

Biological Process: cellular response to amino acid starvation; cellular response to glucose starvation